Intrinsic features of Zika Virus non-structural proteins NS2A and NS4A in the regulation of viral replication
Fig 8
The ISGs expression induced by NS4A in U251 or HeLa cells.
(a) U251 cells with or without NS4A expression were cultured by DMEM with 2% FBS for 24 h. Total RNA was prepared from the cells for ISG transcription by qRT-PCR. (b) HeLa cells were transfected with plasmids expressing NS4A or empty vector for 24 h. After the cells were cultured in DMEM with 2% FBS for 24 h, total RNA was prepared from the cells for ISG transcription by qRT-PCR. The differences between groups of control and NS4A were evaluated by two-tailed Student’s t test. Data are means ± SEM triplicate experiments; *, P< 0.05; **, P< 0.01; ***, p < 0.001.