Intrinsic features of Zika Virus non-structural proteins NS2A and NS4A in the regulation of viral replication
Fig 5
NS2A and NS4A broadly inhibited viral infection.
(a-c & e-h) The antiviral activity of NS2A and NS4A in HMC3 or U251 cells. HMC3 or U251 cells expressing NS2A or NS4A were infected by 0.1 MOI of ZIKV/SZ01, ZIKV/MR766, SFTSV or EV-A71 for 24 and 48 h. Viral RNA copies in infected cells were determined by qRT-PCR. (d & i-l) Antiviral activity of NS2A and NS4A in HEK293 or HeLa cells. HEK293 or HeLa cells were transfected with plasmids expressing NS2A or NS4A for 24 h, followed by infection with ZIKV/SZ01, ZIKV/MR766, SFTSV or EV-A71 for 24 (Fig 5D) or 48 h. Viral RNA copies in infected cells were determined by qRT-PCR. The differences between groups of control and NS2A or NS4A were evaluated by two-tailed Student’s t test. Data are means ± SEM of triplicate experiments; *, P < 0.05; **, P< 0.01; ***, p < 0.001.