Intrinsic features of Zika Virus non-structural proteins NS2A and NS4A in the regulation of viral replication
Fig 1
NS4A protein occurred later than NS2B, NS3 and NS5 during ZIKV infection.
(a-b) Replication of ZIKV genome in microglial cells (HMC3) and astrocytic cells (U251). (c-e) Expression of ZIKV proteins in HMC3, U251 and Vero cells. (f-h) Gray scale analysis of Fig 1C (f), Fig 1D (g) and Fig 1E (h). After ZIKV/SZ01 (0.1 MOI) infection of HMC3, U251 or Vero cells for 0, 6, 18, 30, 42, and 54 h, the viral RNA or viral proteins in cell lysates was determined by qRT-PCR or western blot analysis. Data are means ± SEM of triplicate experiments. E, envelope protein; NS2B, non-structural 2B protein; NS3, non-structural 3 protein; NS4A, non-structural 4A protein; NS5, non-structural 5 protein.