Transplacental Zika virus transmission in ex vivo perfused human placentas
Fig 2
Detection of ZIKV RNA in chorionic villi of placentas perfused with ZIKV.
ZIKV RNA was detected with ISH in formalin fixed, paraffin embedded tissue of perfused placentas. Chromogenic staining for cytokeratin-7 (cyto-7) and CD163 was performed in sequential slides to detect trophoblasts and Hofbauer cells, respectively. A: Staining for ZIKV RNA, trophoblasts and Hofbauer cells in a placenta that was perfused for 40 minutes with ZIKVBPL+DENV nAbs. B: Staining for ZIKV RNA, trophoblasts and Hofbauer cells in a placenta that was perfused for 120 minutes with ZIKVBPL+flavivirus naïve serum. C: Staining for ZIKV RNA in a placenta that was obtained directly after birth and was not perfused. Arrows indicate positive signal for ZIKV RNA.