Vaccine-linked chemotherapy with a low dose of benznidazole plus a bivalent recombinant protein vaccine prevents the development of cardiac fibrosis caused by Trypanosoma cruzi in chronically-infected BALB/c mice
Fig 5
Effect of vaccine-linked chemotherapy on antigen-specific CD4+ and CD8+ T cells memory response.
Mononuclear cells isolated from mice at 200 days p.i. were co-cultivated with macrophages stimulated in vitro with TSA-1-C4+Tc24-C4 (25 μg/mL) for 96 h. Data were analysed using FlowJo X software. Stimulation index of antigen-specific (A) CD4+ Central Memory, (B) CD8+ Central Memory, (C) CD4+ Effector Memory and (D) CD8+ Effector Memory T cells are presented. Each point represents an individual mouse, horizontal lines denote mean ± SD or median ± interquartile ranges values according to the normality of the data. Data were analysed using Student´s t-test or Mann-Whitney U-test. Significance is indicated as follows *, P≤0.05.