Ameliorative effects of Schisandrin B on Schistosoma mansoni-induced hepatic fibrosis in vivo
Fig 5
Sch B inhibits apoptosis in S. mansoni-infected mice.
(A) RNA transcription levels of apoptotic markers. Data are presented as mean ± S.D. (n = 9–11). (B) Representative western blot images showing protein levels of apoptotic markers. (C) Protein expression levels of apoptotic markers, relative to that of α-tubulin. Results are presented as mean ± S.D. (n = 5). (D) Representative plots showing Annexin V-FITC and PI double staining, measured by flow cytometry. Viable cells are shown in the bottom left quadrant (double negative); early apoptotic cells are shown in the bottom right quadrant (Annexin V positive and PI negative); late apoptotic cells are shown in the upper right quadrant (double-positive). (E) Percentages of viable cells, shown as the mean ± S.D. (n = 5). (F) Percentages of apoptotic cells are shown as the mean ± SD (n = 5). (G) Representative microphotographs of TUNEL staining, shown at 200× and 400× magnifications. Scale bar corresponds to 100 μm. (H) Quantification was performed on three slides in each group. Ten random microscopic fields were counted on each slide. * P-value < 0.05, *** P-value < 0.001, and **** P-value < 0.0001 compared with control group; # P-value < 0.05, ## P-value < 0.01, and ### P-value <0.001 compared with infected group.