Relevance of oxidative stress in inhibition of eIF2 alpha phosphorylation and stress granules formation during Usutu virus infection
Fig 5
USUV infection inhibits ArsNa-induced SGs formation.
(A) Vero cells were mock- or USUV- infected at an MOI of 1, treated with ArsNa (0.5 mM), BTdCPU (20μM), DTT (1mM) or BEPP (10 μM) for 4 hours at 24 and 48 h p.i., and analyzed by immunofluorescence. SGs were detected with anti-G3BP1 antibody (green) and USUV-infected cells with anti-dsRNA antibody (red). Nuclei were stained with To-Pro-3 (blue). (B) Quantification of SG-positive cells in cells treated as in A. Scale bars, 25 μm. Statistically significant differences were considered when P<0.05 and marked with an asterisk.