Epigenetic regulation of defense genes by histone deacetylase1 in human cell line-derived macrophages promotes intracellular survival of Leishmania donovani
Fig 2
Analysis of histone promoter acetylation and HDAC1 occupancy pattern of host defense genes in response to Leishmania donovani infection.
THP-1 cells (105 cells/ml) were infected with L. donovani. At 6 and 24 h post-infection, the histone acetylation pattern on the defense genes promoter was analysed by ChIP using antibodies specific for H3 acetylation A: 6 h post infection B: 24 h post-infection. HDAC1 occupancy at the defense genes promoters was also determined by ChIP using antibodies specific for HDAC1 C: 6 h post-infection D: 24 h post-infection. The immunoprecipitated DNA fragments were quantitated by qRT-PCR using primers specific for the promoter region of each of the host genes studied. The changes in the level of H3-acetylation and HDAC1 occupancy are depicted as fold change over uninfected cells (UI). Basal levels of acetylation and HDAC1 occupancy of uninfected cells were used for data normalization and assessed relative abundance. Numbers >1 indicates up-regulation, and <1 indicates down regulation of host genes in infected cells as compared to uninfected cells. The results are mean ± SD (n = 3).