Epigenetic regulation of defense genes by histone deacetylase1 in human cell line-derived macrophages promotes intracellular survival of Leishmania donovani
Fig 1
Expression of host defense genes in L. donovani infected THP-1 cells.
THP-1 cells (105 cells/ml) were treated with PMA (50 ng/ml for 48 h), as explained in the methods section. Uninfected and infected cells were harvested at A: 6 h and B: 24 h post-infection. RNA was extracted from infected and uninfected THP-1 cells, and expression of defense genes was quantitated by qRT-PCR. Gene expression changes are depicted as fold change in transcription compared to that of uninfected cells (UI). Numbers >1 indicates up-regulation, and <1 indicates the down-regulation of host genes in infected cells as compared to the uninfected cells. RNU6A was used as the housekeeping gene. Data analysis was performed using a 2^-ΔΔCT method. The basal level of transcript expression of uninfected cells was used for data normalization and to assess relative abundance. The results represent the mean ± SD (n = 3).