2'-Hydroxyflavanone activity in vitro and in vivo against wild-type and antimony-resistant Leishmania amazonensis
Fig 7
Resistance confirmation in in vivo recovered promastigotes.
L. amazonensis promastigotes were recovered from the in vivo limiting dilution experiment from each treated group and cultivated with Schneider’s Drosophila medium. Promastigotes were incubated in the presence or absence of the potassium antimony tartrate (SbIII) (0.3–5000 μM) for 72 hours. The viability was measured by resazurin. The IC50 for resistance confirmation was calculated via nonlinear regression using GraphPad Prism 6.0. The values are presented as the mean ± standard error of two different experiments. Panel A: Wild-type promastigotes; Panel B: Promastigotes recovered from the vehicle treatment group; Panel C: Promastigotes recovered from the 2HF-treated group; Panel D: Promastigotes recovered from the meglumine antimoniate-treated group.