Genetic manipulation of Leishmania donovani threonyl tRNA synthetase facilitates its exploration as a potential therapeutic target
Fig 6
Effect of borrelidin on the rLdThrRS enzyme.
(A) 2D structure of borrelidin (B) Dose-response inhibition of the aminoacylation activity of rLdThrRS in the presence of known ThrRS inhibitor, borrelidin. Inhibitor concentrations are plotted in the log scale on X-axis. The experiment was performed with 0.001–10 μM borrelidin. (C) Top Panel: ITC profile for binding of rLdThrRS to borrelidin indicating the sequential injection of the drug into rLdThrRS after correction of the heat of dilution of the drug. Bottom panel: Plot of integrated heat data fitted to a one-site model at 25°C. (D) Bar diagram is describing the variation of the magnitude of thermodynamic parameters of binding of borrelidin to rLdThrRS.