Genetic manipulation of Leishmania donovani threonyl tRNA synthetase facilitates its exploration as a potential therapeutic target
Fig 5
Cell cycle and morphological study of heterozygous parasites.
Cell cycle analysis of (A) WT, (B) heterozygous parasites (ThrRS/NEO) and (C) Rescue mutants (ThrRS/NEO[pThrRS+]). PI fluorescence (FL2-Area) is plotted versus cell count. The first peak (red color) indicates cells in G0/G1 phase, S phase is indicated in blue color, and third peak (red color) represents cells in G2/M phase. (D) A bar graph is representing the percentage of cells in the G0/G1 phase of WT, ThrRS/NEO and ThrRS/NEO[pThrRS+] parasites. The results represent mean ± S.D with n = 3, ***P < 0.005 statistical difference from the wild-type control. (E) Confocal imaging of WT, ThrRS/NEO and ThrRS/NEO[pThrRS+] parasites.