Selective inhibition of RNA polymerase I transcription as a potential approach to treat African trypanosomiasis
Fig 8
Incubation of T. brucei with Pol I transcription inhibitors leads to rapid disintegration of nucleoli.
The nucleolus can be visualised with the L1C6 nucleolar marker as one spot in T. brucei in the G1 cell cycle stage. Nucleoli rapidly disintegrate into multiple L1C6 positive spots after incubation of cells with 3 μM quarfloxin (A), BMH-21 (B), CX-5461 (C) or 800 nM suramin (D). Each dot indicates a cell, where the number of L1C6 positive spots is indicated. The mean indicated with a line, and standard deviation with error bars. A total of 100 cells was analysed (two biological replicates of ~50 cells).