Selective inhibition of RNA polymerase I transcription as a potential approach to treat African trypanosomiasis
Fig 5
Incubation of T. brucei with Pol I transcription inhibitors leads to rapid disappearance of the Pol I Expression Site Body (ESB) and fragmentation of the nucleolus.
Immunofluorescence analysis of T. brucei TY-YFP-RPA2 incubated with 3 μM quarfloxin, BMH-21, CX-5461 or 800nM suramin for three hours (h). The panels show representative T. brucei cells in the G1 cell cycle stage with DNA visualised with the DNA stain DAPI, and the nucleolus with the L1C6 nucleolar marker. The Pol I complex can be visualised using Yellow Fluorescence Protein (YFP), as one allele of the RPA2 gene (second largest Pol I subunit) is epitope tagged. Signal corresponding to the extra-nucleolar ESB is indicated with a white arrow head. Scale bar indicates 5 μm.