Selective inhibition of RNA polymerase I transcription as a potential approach to treat African trypanosomiasis
Fig 3
Irreversible inhibition of T. brucei growth after incubation with quarfloxin or CX-5461 as determined using wash-out assays.
T. brucei SM cells were treated with various concentrations of quarfloxin (A), BMH-21 (B) or CX-5461 (C) for two hours, washed, diluted into fresh media and cell growth was monitored. Untreated cells, and parasites incubated with the appropriate drug vehicle (DMSO for quarfloxin and BMH-21) or NaH2PO4 (for CX-5461) were used as controls. The mean of three biological replicates is plotted with the standard deviation indicated with error bars.