Selective inhibition of RNA polymerase I transcription as a potential approach to treat African trypanosomiasis
Fig 1
RNA polymerase I (Pol I) transcription inhibitors selectively inhibit proliferation of bloodstream form Trypanosoma brucei.
An Alamar Blue in vitro cytotoxicity assay was used to determine sigmoidal dose response curves of T. brucei SM (A-D) or the MCF10A human breast epithelial cell line (E-H) incubated with the Pol I inhibitors quarfloxin (A, E), BMH-21 (B, F), CX-5461 (C, G) or the anti-trypanosomal agent suramin (D, H) [44]. The mean percentage of signal relative to the negative control (vehicle without drug) from three biological replicates is plotted with the standard deviation indicated with error bars.