Iminosugars Inhibit Dengue Virus Production via Inhibition of ER Alpha-Glucosidases—Not Glycolipid Processing Enzymes
Fig 3
NB-DNJ reduces glycolipid production in a potent, dose-dependent manner in primary human MDMΦs.
Uninfected MDMΦs were treated with a titration of NB-DNJ for 48 hours, and glycolipids were isolated from whole cell lysates. Production of monosialodihexosylganglioside (GM3) was normalized to total protein content for each sample, and each treatment was normalized to untreated controls on a donor-specific basis. NB-DNJ inhibited production of GM3 in a dose-dependent manner that was approximately 10-fold more potent than the antiviral inhibition. Data are presented as mean ± SD from assay of three biological replicates assayed in technical singlicate.