Reciprocity between Regulatory T Cells and Th17 Cells: Relevance to Polarized Immunity in Leprosy
Fig 5
Effect of Th17 cytokines on FoxP3+Treg cells in BL/LL.
A) PBMCs were cultured for 72 h in different conditions with or without the presence of cytokines inducing Th17 population such as TGF-β, IL-6, IL-23 and those secreted by Th17, IL-17 and IL-22 in different wells and readout was measured in terms of FoxP3+ on CD4+CD25+ cells on one hand and simultaneous IL-17 production by CD4+T cells. B) Bar graphs show the cumulative data in BL/LL (n = 5) of FoxP3+ expression on CD4+ CD25+ cells as against C) IL-17 production by CD4+T cells under the same conditions as mentioned above. Mean with SEM are shown in each bar while P value< 0.05 was considered to be significant. Total number of cells analyzed by flow cytometry were 500,000. Data analysis was performed with flowjo software. Statistical analysis was done using Student’s t test for unpaired samples.