Reciprocity between Regulatory T Cells and Th17 Cells: Relevance to Polarized Immunity in Leprosy
Fig 2
Identification of Th17 cells in Leprosy.
A) Representative flow cytometric analysis showing IL-17 production on gated CD4+ CD45RO+ memory T cells following in vitro stimulation with M. leprae (WCL) vs. unstimulated for 48 h in a Leprosy patient with BT. 6 h PMA stimulation was used as positive control. B) Scatter plots are showing cumulative IL-17 production in gated CD4+ CD45RO+ cells in different groups of Leprosy patients (BT/TT vs. BL/LL) and Healthy Contacts (n = 10). Each dot represents a single individual of BT/TT (n = 15) and BL/LL (n = 15) patient. C) Scatter graphs are showing CCR6 surface expression on gated CD4+CD45RO+ cells in different groups of Leprosy patients (BT/TT vs. BL/LL) (n = 10). Median values are shown in each set while P value< 0.05 was considered to be significant. Total number of cells analyzed by flow cytometry were 500,000. Data analysis was performed with flowjo software. Statistical analysis was done using Student’s t test for unpaired samples.