Functional Analysis of the Unique Cytochrome P450 of the Liver Fluke Opisthorchis felineus
Fig 5
Kaplan-Meier survival curves and pentoxyresorufin metabolizing activity in worm tissues after RNA interference.
A. A set of Kaplan-Meier survival curves (out of three independent experiments) is shown. Statistical difference in survival log-rank (Mantel-Haenszel) test between each pair of samples was calculated. The survival curves show significance when either the wild type (p<0.0001) or LUC (p<0.001) or mock control (p = 0.002) is compared to CYP group and no difference is observed when LUC group and mock control are considered (p = 0.7)('survival'(v.2.38) R package). B. Analysis of pentoxyresorufin (PR) metabolizing activity. Worms five days after the knockdown were treated for 20 h with pentoxyresorufin, and images were acquired using multiple fluorescence filters using optical sectioning by means of the AxioImager fluorescence microscope (Zeiss). The total size of the resorufin particles in each worm was measured. Data were expressed as a fold difference compared to the wild-type (not exposed to dsRNA) control. Data are presented as means ± S.D. Results are averaged from three independent experiments. Significance at p < 0.005, ***; p < 0.01, ** (F-test).