Molecular Diagnosis of Pathogenic Sporothrix Species
Fig 5
Sporothrix brasiliensis distribution in mouse organs at 10 days post infection.
Mice were challenged with intravenous injections of 1×106 yeast cells (S. brasiliensis—CBS 132990). Gel electrophoresis (1.2% agarose) show PCR products (5-μL aliquots) amplified with primers, Sbra-F and Sbra-R (469 bp; yellow). Template DNAs were extracted and purified directly from fresh tissues (n = 5) dissected from (left-to-right, top-to-bottom) spleen, lungs, liver, kidneys, heart, brain, tail, and feces. Control reactions (347 bp; purple) used primers that recognized β-actin of the mouse genome. For PCR assays of DNA extracted from feces, the inhibition control was assessed by spiking the reaction solution with genomic DNA (100 ng) extracted from a S. brasiliensis culture (CBS 132990). Statistical analyses of the diagnostic accuracies are shown in S2 Table.