A Madurella mycetomatis Grain Model in Galleria mellonella Larvae
Fig 4
Grain formation in human, mouse and larvae of Galleria mellonella.
In panels A, B and C the presence of grains in tissue is shown. In panel A, black grains are clearly visible inside an excised part of the mycetoma lesion of a human patient. In panel B, grain formation inside a Balb/c mouse is shown. In panel C, grains inside a dissected G. mellonella larvae are demonstrated. In panel D, E and F, 100 times magnified HE slides of mycetoma grains show that the grains are surrounded with immune cells, and a large zone of neutrophils is present in both human and mice. In G. mellonella larvae these immune cells are not present yet (panel F) at a time point of 3 days after infection. In grains obtained from human, mouse and G. mellonella larvae fungal hyphae can be seen as demonstrated with Grocott staining (panels G, H, I). With Sirius Red staining a clear encapsulation of grains with collagen is seen in human, mouse and G. mellonella larvae (panels J, K, L).