A Madurella mycetomatis Grain Model in Galleria mellonella Larvae
Fig 3
Melanization of Galleria mellonella larvae during M. mycetomatis infection.
In panel A, three larvae infected with 0.04, 0.4 and 4 mg mm55 are shown. As is seen, the larvae infected with 0.04 mg mm55 is not melanised (left larvae), the larvae infected with 0.4 mg mm55 is slightly melanised (middle larvae) and the larvae infected with 4 mg mm55 is heavily melanised (right larvae). In panels B, C and D, the melanization of the haemolymph is demonstrated by measuring the OD405nm of the haemolymph at time points; 4h, 24h, 72h, 168h and 700h after infection. In panel B, the melanization of larvae infected with mm55 is shown, in panel C those infected with mm68 and in panel D those infected with cn796. At all time-points it appeared that the larvae infected with the highest inoculum of M. mycetomatis were the most melanised, those with the lowest inoculum, were the least melanised.