Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Figure 3
A) Immunodiffusion tests with different CFAs from 12 strains of Paracoccidioides lutzii.
1 = EPM 147; 2 = EPM 148; 3 = EPM 196; 4 = EPM 201; 5 = EPM 205; 6 = EPM 206; 7 = EPM 208; 8 = EPM 212; 9 = EPM 213; 10 = EPM 223; 11 = EPM 209; 12 = EPM 226. In the center well, S* = P. lutzii serum (gold standard). P. lutzii strains EPM 227 and EPM 228 were positive (not shown). B) Immunodiffusion test showing that S* = P. lutzii gold standard serum do not react with CFA from P. brasiliensis Pb18 and B339. C) Immunodiffusion test using Ag Exo (crude exoantigen of P. lutzii) and Ag TCA (crude exoantigen of P. lutzii precipitated with trichloroacetic acid) versus P. lutzii serum (S* = P. lutzii gold standard serum and S = suspected P. lutzii serum). Observe the weak band in both cases. D) immunodiffusion test showing that S* (P. lutzii gold standard serum) do not react with Ag Exo, Ag TCA and CFA from P. brasiliensis B339 antigen. E) Immunodiffusion test of 12 sera from patients with PCM due to P. lutzii using P. lutzii CFA. In the center well is the CFA antigen (Ag), and different PCM sera are in the peripheral wells (1 to 12). F) Immunodiffusion test showing that S* (P. lutzii gold standard serum) do not react with Exo or CFA of P. brasiliensis B339; and SPb (serum of PCM patient due to P. brasiliensis) do not react with Exo or CFA of P. lutzii.