Mechanisms of Pyrethroid Resistance in the Dengue Mosquito Vector, Aedes aegypti: Target Site Insensitivity, Penetration, and Metabolism
Figure 5
Real time quantitative PCR analysis of selected genes from the microarray experiments.
Transcription levels of seven P450 genes were individually validated in adult female (A), adult male (B), and fourth instar larvae (C) in SP and SMK strains of Aedes aegypti. Ribosomal protein S3 gene (RPS3) was used to normalize the data. Expression ratios (SP/SMK) are expressed in parentheses. (D) Correlation between the microarray and real time quantitative PCR (R2 = 0.824). (E) Gene copy number of 7 P450s and RPS3 quantified by real time PCR. The ratios of relative copy number (SP/SMK) are expressed in parentheses. (F) Gene clusters of cytochrome P450 and the CYP9M subfamily on the A. aegypti supercontig 1.29 on the 2nd chromosome. The error bars represent standard errors of three (A, B, and C) and eight (E) biological replicates.