Proteomic Analysis of the Excretory-Secretory Products from Larval Stages of Ascaris suum Reveals High Abundance of Glycosyl Hydrolases
Figure 5
qPCR and glucosidase hydrolytic activity analysis of GH31 proteins.
A. A qPCR analysis for two GH31 proteins on cDNA produced from different larval stages and adult worm tissues. B. Comparison of the glucosidase hydrolytic activity in water-soluble (white bars) and water-insoluble extract (black bars) from different adult tissues. Results are shown as average + SD. Substrates used in the assays were lactose, maltose and sucrose. (Int F: female intestine; Int M: male intestine; Rep F: female reproductive system; Rep M: male reproductive system; Cut F: female cuticle; Cut M: male cuticle). (* P<0.05).