Identification and Functional Analysis of Trypanosoma cruzi Genes That Encode Proteins of the Glycosylphosphatidylinositol Biosynthetic Pathway
Figure 8
Cell membrane mucins in T. cruzi GPI8 mutants.
Immunoblot of total (T), cytoplasmic (C) and membrane (M) fractions of WT epimastigotes, TcGPI8 single allele knockout, neomycin resistant (+/−N2) and double resistant TcGPI8 (N/H2) mutant cell lines. Equivalent amounts of protein from each fraction, as showed by the Coomassie blue stained bands (bottom panel), were transferred to nitrocellulose membranes and incubated with anti-mucin antibodies and revealed with horseradish peroxidase conjugated secondary antibodies.