Identification and Functional Analysis of Trypanosoma cruzi Genes That Encode Proteins of the Glycosylphosphatidylinositol Biosynthetic Pathway
Figure 7
Cell membrane morphology of T. cruzi GPI8 mutants.
Transmission electron microscopy showing cellular membranes of wild type T. cruzi epimastigotes (WT), TcGPI8 single allele knockout, neomycin resistant (+/− N1) and double resistant TcGPI8 epimastigote mutants (N/H1). Although displaying similar morphologies, representative images show that single allele TcGPI8 mutants present a thinner layer of parasite glycocalyx, when compared to wild type cells, whereas cell membranes of double resistant parasites present a glycocalyx layer that is slightly thicker than the glycocalyx of wild type parasite membranes (indicated by the arrows).