Identification and Functional Analysis of Trypanosoma cruzi Genes That Encode Proteins of the Glycosylphosphatidylinositol Biosynthetic Pathway
Figure 3
Cellular localization of T. cruzi enzymes of the GPI biosynthetic pathway.
Epimastigotes were transiently transfected with the plasmids pTREX-TcDPM1-GFP (A), pTREX-TcGPI3-GFP (B), pTREX-TcGPI12-GFP (C) or pTREXnGFP as a control plasmid (D) and (E). Transfected parasites were fixed with 4% paraformaldehyde, incubated with the ER marker anti-BiP (1∶1000) and the secondary antibody conjugated to Alexa 555 (1∶1000). Cells were also stained with DAPI showing the nuclear and kinetoplast DNA. In panel E, parasites that were not incubated with the primary, anti-BiP antibody are shown as negative controls. Images were captured with the Nikon Eclipse Ti fluorescence microscope. Scale bars: 5 µm.