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Scabies Mite Peritrophins Are Potential Targets of Human Host Innate Immunity

Figure 2

Specificity of mouse antisera raised against a recombinant chitin binding domain of SsPTP1.

Shown is a series of purified scabies mite proteins on a coomassie-stained SDS-PAGE (A) and a corresponding western with a peritrophin-specific antibody (B). The gel (A) demonstrates the purity of the recombinant peptide CBD1 (lane 2) and the fusion protein TSP-CBD1 (lane 3) and (B) the specificity of the antibody raised against CBD1, confirming no cross-reaction with unrelated scabies mite proteins SMSB3a (lane 1), SMIPP-S D1 (lane 5), SMIPP-S I1 (lane 6) and BSA (lane 4).

Figure 2

doi: https://doi.org/10.1371/journal.pntd.0001331.g002