Structural Optimization and De Novo Design of Dengue Virus Entry Inhibitory Peptides
Figure 5
Peptide:E protein binding assay.
Biolayer interferometry was used to assay the binding of the peptides to truncated E Protein. The association and dissociation of increasing concentrations of truncated E protein to peptides DN57opt (A) and 1OAN1 (B) are shown. A buffer blank (PBS, 0.02% Tween-20, 0.1% BSA) containing no E protein was run for each peptide. The affinity of the peptides for the truncated E protein was calculated (DN57opt KD = 1.2×10−6±0.6×10−6 M (mean±sd), 1OAN1 KD = 4.5×10−7±2.0×10−7 M).