Detection of Mycolactone A/B in Mycobacterium ulcerans–Infected Human Tissue
Figure 5
Cytotoxicity of mycolactone on HELF cells.
A and B show the effect of ASL from Mu infected skin on HELF cells after 48 h with detachment of cells from culture plate, numerous apoptotic cells (orange nuclei stained with acridine orange) and few live spindle shaped cells (green nuclei with ethidium bromide). A and B represent ASL from a nodular lesion and an ulcerative lesion respectively. C shows the effect of purified mycolactone at a concentration of 5 µg/ml and D is a negative control demonstrating the effect of ASL from uninfected human skin. Pictures taken at x10 with a DM1 6000B Leica fluorescent microscope.