Development of Highly Organized Lymphoid Structures in Buruli Ulcer Lesions after Treatment with Rifampicin and Streptomycin
Figure 8
Follicle-like lymphocyte foci reveal organized cellular formation.
Serial sections of a representative lymphocyte focus stained with HE (A; magnification ×40) or with antibodies against different cellular markers and haematoxylin as counterstain (B to I; magnification ×100). (A) Large aggregate of lymphocytes in the upper dermal layer between two venules. Square marks area of magnification chosen for pictures B to I. (B) CD20+ B lymphocytes build the most abundant cell subset. (C, D, E) Staining against CD3, CD4 and CD8, respectively, exposes also high loads of T lymphocytes with T helper clearly being more prevalent than cytotoxic T cells. (F) APCs (CD68+) are scattered throughout the entire structure. (G) Activated lymphocytes appear positive for CD45RO staining. (H) S100+ dermal dendrocytes (dDC) lie in between lymphocytes. (I) Ki67 staining demonstrates certain proliferation of cells and occasional appearance of discrete spots of hyperproliferation (insert).