Development of Highly Organized Lymphoid Structures in Buruli Ulcer Lesions after Treatment with Rifampicin and Streptomycin
Figure 7
Composite of mixed cellular infiltrates.
Histological sections representative for four patients stained with antibodies against different cellular markers (counterstain haematoxylin). Magnifications at ×40 (A, E, G), ×100 (C, D, F, I, J), ×200 (Ainsert, B, H) or ×1000 (Einsert). (A) Only few scattered PMNL staining positive for Elastase were found within cellular infiltrates. (Ainsert, B) Neutrophilic and NK cell (CD56+) foci inside necrotic areas display signs of advanced apoptosis. (C) Lymphocytes mainly expose a CD3+ phenotype. (D) Small focal CD20+ lymphocyte spots are scattered through infiltrates. (E) Staining against CD14 illustrates large numbers of histiocytes enclosing necrotic tissue shedding massive amounts of sCD14 (insert). (F) Levels of epidermal CD1a+ Langerhans cells are remarkably elevated compared to healthy skin. (G) Aggregation of S100+ dDCs near necrotic spots. (H) Elongated cellular appendices of dDCs reach into the necrotic tissue. (I) Large numbers of lymphocytes are CD45RO+. (J) Same area as in (I). Proliferating lymphocytes are highly Ki67+.