Development of Highly Organized Lymphoid Structures in Buruli Ulcer Lesions after Treatment with Rifampicin and Streptomycin
Figure 6
Detailed organization of granulomas.
Histological serial sections representative for four patients were stained with antibodies against different cellular surface or cytoplasmic markers (counterstain haematoxylin). Magnification ×40 (I), ×100 (A, B, C, E, F, G, H, Iinsert), ×200 (D) and ×400 (Finsert). (A, B, C) Staining with CD3, CD4 and CD8, respectively, reveals a belt of helper as well as cytotoxic T lymphocytes surrounding the APC core. (D) S100+ dermal dendrocytes (dDC) spread among T lymphocytes in the outer layer of a granuloma. (E) Focus of CD20+ B lymphocytes at the border of a granuloma. (F) CD68+ APC in the centre of a representative granuloma; insert shows large Langhans' giant cell. (G) Remarkable large amounts of membrane bound (arrow) and soluble (arrowhead) CD14 can be observed. (H) Distribution of activated CD45RO+ lymphocytes. (I) Proliferating Ki67+ cells indicate the active status of granulomas.