Development of a qPCR assay for Fasciola spp. identification and a deep amplicon sequencing method for differentiation of fluke species in UK livestock

• Muhammad Abbas,

  Roles Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

  Affiliation Discipline of Comparative Biomedical Sciences, School of Veterinary Medicine, University of Surrey, Guildford, United Kingdom

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• Kezia Kozel,

  Roles Investigation, Methodology, Resources, Writing – review & editing

  Affiliation Discipline of Comparative Biomedical Sciences, School of Veterinary Medicine, University of Surrey, Guildford, United Kingdom

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• Olukayode Daramola,

  Roles Supervision, Writing – review & editing

  Affiliation School of Veterinary Medicine, University of Lancashire, Preston, United Kingdom

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• Nick Selemetas,

  Roles Supervision, Writing – review & editing

  Affiliation Discipline of Microbes, Infection and Immunity, School of Veterinary Biosciences, University of Surrey, Guildford, United Kingdom

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• Qasim Ali,

  Roles Resources, Writing – review & editing

  Affiliation Department of Pathobiology, University of Veterinary and Animal Sciences, Swat, Pakistan

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• Shoaib Ashraf,

  Roles Resources, Writing – review & editing

  Affiliations Department of Pathobiology, College of Veterinary Medicine, Riphah International University, Lahore, Pakistan, Department of Biomedical Sciences, Ross University, School of Veterinary Medicine (RUSVM), St Kitts and Nevis, West Indies

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• Ibrahim Isah,

  Roles Resources, Writing – review & editing

  Affiliation Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

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• Inaki Deza-Cruz,

  Roles Resources, Writing – review & editing

  Affiliation The Royal (Dick) School of Veterinary Studies and The Roslin Institute, The University of Edinburgh Easter Bush Veterinary Centre, Midlothian, Edinburgh, United Kingdom

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• Sai Fingerhood,

  Roles Resources, Writing – review & editing

  Affiliation Department of Veterinary Pathology, University of Nottingham, Nottingham, United Kingdom

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• Mark W. Robinson,

  Roles Resources, Writing – review & editing

  Affiliation School of Biological Sciences, Queen’s University, Belfast, United Kingdom

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• Eric R. Morgan,

  Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

  Affiliation School of Biological Sciences, Queen’s University, Belfast, United Kingdom

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• Umer Chaudhry ,

  Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

  * E-mail: m.betson@surrey.ac.uk (MB); umer.chaudhry@liu.edu (UC)

  Affiliation Department of Veterinary Biomedical Sciences, Lewyt College of Veterinary Medicine, Long Island University, Brookville, New York, United States of America

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• Martha Betson

  Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

  * E-mail: m.betson@surrey.ac.uk (MB); umer.chaudhry@liu.edu (UC)

  Affiliation Discipline of Comparative Biomedical Sciences, School of Veterinary Medicine, University of Surrey, Guildford, United Kingdom

  https://orcid.org/0000-0002-4220-3290

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