PvGTSeq and PvCRiSP: Two amplicon-based targeted sequencing panels for Plasmodium vivax

Paulo C. Manrique-Valverde; Chloe M. Hasund; Katrina A. Kelley; Jorge-Eduardo Amaya-Romero; Myriam Arévalo-Herrera; Raphael Brosula; Jose E. Calzada; Stella M. Chenet; Vladimir Corredor; Angela M. Early; Gustavo Fontecha; David A. Forero-Peña; Sócrates Herrera; Justin T. Lana; Margaret Laws; Reza Niles-Robin; Nicanor Obaldia; Ana M. Santamaria; Philipp Schwabl; Sarah Auburn; Daniel E. Neafsey

doi:10.1371/journal.pntd.0013663

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Open Access

Peer-reviewed

Research Article

PvGTSeq and PvCRiSP: Two amplicon-based targeted sequencing panels for Plasmodium vivax

Paulo C. Manrique-Valverde,

Roles Conceptualization, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliations Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America, Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, United States of America

https://orcid.org/0000-0003-3412-0249

⨯
Chloe M. Hasund,

Roles Conceptualization, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

Affiliations Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America, Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, United States of America
⨯
Katrina A. Kelley,

Roles Data curation, Investigation, Methodology, Project administration, Resources, Validation, Writing – review & editing

Affiliations Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America, Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, United States of America
⨯
Jorge-Eduardo Amaya-Romero,

Roles Software, Writing – review & editing

Affiliations Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America, Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, United States of America
⨯
Myriam Arévalo-Herrera,

Roles Data curation, Writing – review & editing

Affiliations Malaria Vaccine and Drug Development Center, Cali, Colombia, Caucaseco Scientific Research Center, Cali, Colombia
⨯
Raphael Brosula,

Roles Data curation, Software, Writing – review & editing

Affiliation Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, United States of America
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Jose E. Calzada,

Roles Data curation, Writing – review & editing

Affiliations Universidad de Panamá, Panamá, República de Panamá, Departamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud (ICGES), Panamá, República de Panamá
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Stella M. Chenet,

Roles Data curation, Writing – review & editing

Affiliations Instituto de Investigación de Enfermedades Tropicales (IET), Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas (UNTRM), Chachapoyas, Peru, Facultad de Medicina (FAMED), Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas (UNTRM), Chachapoyas, Peru
⨯
Vladimir Corredor,

Roles Data curation, Writing – review & editing

Affiliation Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia
⨯
Angela M. Early,

Roles Software, Writing – review & editing

Affiliation Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, United States of America
⨯
Gustavo Fontecha,

Roles Data curation, Writing – review & editing

Affiliation Instituto de Investigaciones en Microbiología, Facultad de Ciencias, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras
⨯
David A. Forero-Peña,

Roles Data curation, Writing – review & editing

Affiliation Biomedical Research and Therapeutic Vaccines Institute, Ciudad Bolívar, Venezuela
⨯
Sócrates Herrera,

Roles Data curation, Writing – review & editing

Affiliations Malaria Vaccine and Drug Development Center, Cali, Colombia, Caucaseco Scientific Research Center, Cali, Colombia
⨯
Justin T. Lana,

Roles Data curation, Writing – review & editing

Affiliation Clinton Health Access Initiative, Boston, Massachusetts, United States of America
⨯
Margaret Laws,

Roles Data curation, Project administration, Resources, Writing – review & editing

Affiliations Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America, Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, United States of America
⨯
Reza Niles-Robin,

Roles Data curation, Writing – review & editing

Affiliation National Malaria Program, Ministry of Health, Georgetown, Guyana
⨯
Nicanor Obaldia,

Roles Data curation, Writing – review & editing

Affiliations Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America, Departamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud (ICGES), Panamá, República de Panamá
⨯
Ana M. Santamaria,

Roles Data curation, Writing – review & editing

Affiliations Universidad de Panamá, Panamá, República de Panamá, Departamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud (ICGES), Panamá, República de Panamá
⨯
Philipp Schwabl,

Roles Software, Writing – review & editing

Affiliations Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America, Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, United States of America, Institute of International Animal Health/One Health, Friedrich-Loeffler-Institut, Greifswald (Insel Riems), Germany
⨯
Sarah Auburn,

Roles Conceptualization, Funding acquisition, Writing – review & editing

Affiliations Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia, Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom, Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand‌‌
⨯
[ ... ],
Daniel E. Neafsey

Roles Conceptualization, Funding acquisition, Supervision, Writing – original draft, Writing – review & editing

* E-mail: paulomanrique@g.harvard.edu

Affiliations Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America, Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, United States of America
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PvGTSeq and PvCRiSP: Two amplicon-based targeted sequencing panels for Plasmodium vivax

Paulo C. Manrique-Valverde,
Chloe M. Hasund,
Katrina A. Kelley,
Jorge-Eduardo Amaya-Romero,
Myriam Arévalo-Herrera,
Raphael Brosula,
Jose E. Calzada,
Stella M. Chenet,
Vladimir Corredor,
Angela M. Early

Published: May 14, 2026
https://doi.org/10.1371/journal.pntd.0013663

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