Development and evaluation of a triplex real-time PCR assay for enhanced plague diagnostics in Madagascar

• Beza Ramasindrazana ,

  Roles Conceptualization, Data curation, Methodology, Writing – original draft, Writing – review & editing

  * E-mail: rbeza@pasteur.mg

  ¶☯ Equal contribution.

  Affiliations Plague Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Faculté des Sciences, Université d’Antananarivo, Antananarivo, Madagascar

  https://orcid.org/0000-0002-8838-949X

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• Zoé A. Rahalison ,

  Contributed equally to this work with: Zoé A. Rahalison, Philippe Gauthier

  Roles Data curation, Formal analysis, Methodology, Writing – original draft, Writing – review & editing

  ¶☯ Equal contribution.

  Affiliations Plague Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Faculté de Médecine Humaine, Université d’Antananarivo, Antananarivo, Madagascar

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• Philippe Gauthier ,

  Contributed equally to this work with: Zoé A. Rahalison, Philippe Gauthier

  Roles Conceptualization, Formal analysis, Methodology, Supervision, Writing – original draft, Writing – review & editing

  ¶☯ Equal contribution.

  Affiliation CBGP, IRD, INRAE, CIRAD, Institut Agro, Université de Montpellier, Montpellier, France

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• Guillain Mikaty,

  Roles Validation, Writing – original draft, Writing – review & editing

  Affiliation Institut Pasteur, Université Paris Cité, Unité Environnement et Risques infectieux, Cellule d’Intervention Biologique d’Urgence, Paris, France

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• Zaina I. Bodoarison,

  Roles Data curation, Investigation, Writing – review & editing

  Affiliation Plague Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar

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• Lanto A. Maminirina,

  Roles Data curation, Investigation, Writing – review & editing

  Affiliations Plague Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Faculté des Sciences, Université d’Antananarivo, Antananarivo, Madagascar

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• Soloandry Rahajandraibe,

  Roles Data curation, Writing – review & editing

  Affiliation Ministry of Public Health, National Plague Control Program, Antananarivo, Madagascar

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• Mamy G. Randriamanantsoa,

  Roles Data curation, Writing – review & editing

  Affiliation Ministry of Public Health, National Plague Control Program, Antananarivo, Madagascar

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• Gilbert Kayoko,

  Roles Conceptualization, Supervision, Writing – review & editing

  Affiliation World Health Organization, Emergency Preparedness and response, Antananarivo, Madagascar

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• Jean-Claude Manuguerra,

  Roles Methodology, Supervision, Writing – review & editing

  Affiliation Institut Pasteur, Université Paris Cité, Unité Environnement et Risques infectieux, Cellule d’Intervention Biologique d’Urgence, Paris, France

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• Anne-Sophie Le Guern,

  Roles Methodology, Supervision, Writing – review & editing

  Affiliation Institut Pasteur, Université Paris Cité, Yersinia Research Unit, National Reference Centre for Plague and other yersiniosis, Paris, France

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• Andriamiliharison J. Rasamindrakotroka,

  Roles Supervision, Writing – review & editing

  Affiliation Faculté de Médecine Humaine, Université d’Antananarivo, Antananarivo, Madagascar

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• Minoarisoa Rajerison

  Roles Conceptualization, Methodology, Supervision, Validation, Writing – review & editing

  Affiliations Plague Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Faculté des Sciences, Université d’Antananarivo, Antananarivo, Madagascar

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