Assessing anti-rabies vaccine response in humans: A rapid and high-throughput adaptable, pseudovirus-based neutralization assay as an alternative to rapid fluorescent focus inhibition test (RFFIT)

Santhik S. Lupitha; Geetu Rose Varghese; Lekshmi J. Das; Priya Prabhakaran; Ashwini M. Ananda; Reeta S. Mani; Easwaran Sreekumar

doi:10.1371/journal.pntd.0013010

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Open Access

Peer-reviewed

Research Article

Assessing anti-rabies vaccine response in humans: A rapid and high-throughput adaptable, pseudovirus-based neutralization assay as an alternative to rapid fluorescent focus inhibition test (RFFIT)

Santhik S. Lupitha,

Roles Formal analysis, Investigation, Visualization, Writing – original draft

Affiliation Molecular Bioassay Laboratory, Institute of Advanced Virology (IAV), Thiruvananthapuram, Kerala, India
⨯
Geetu Rose Varghese,

Roles Formal analysis, Investigation, Visualization

Affiliation Molecular Bioassay Laboratory, Institute of Advanced Virology (IAV), Thiruvananthapuram, Kerala, India
⨯
Lekshmi J. Das,

Roles Formal analysis, Investigation

Affiliation Molecular Bioassay Laboratory, Institute of Advanced Virology (IAV), Thiruvananthapuram, Kerala, India
⨯
Priya Prabhakaran,

Roles Investigation

Affiliation Department of Virus Applications, Institute of Advanced Virology (IAV), Thiruvananthapuram, Kerala, India
⨯
Ashwini M. Ananda,

Roles Formal analysis, Investigation

Affiliation Department of Neurovirology, National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore, Karnataka, India
⨯
Reeta S. Mani ,

Roles Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

* E-mail: drreeta@gmail.com (RSM); esreekumar@iav.res.in, esreekumar@rgcb.res.in (ES)

Affiliation Department of Neurovirology, National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore, Karnataka, India
⨯
Easwaran Sreekumar

Roles Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

* E-mail: drreeta@gmail.com (RSM); esreekumar@iav.res.in, esreekumar@rgcb.res.in (ES)

Affiliations Molecular Bioassay Laboratory, Institute of Advanced Virology (IAV), Thiruvananthapuram, Kerala, India, Molecular Virology Laboratory, BRIC-Rajiv Gandhi Centre for Biotechnology (BRIC-RGCB), Thiruvananthapuram, Kerala, India

https://orcid.org/0000-0001-6345-3590

⨯

Assessing anti-rabies vaccine response in humans: A rapid and high-throughput adaptable, pseudovirus-based neutralization assay as an alternative to rapid fluorescent focus inhibition test (RFFIT)

Santhik S. Lupitha,
Geetu Rose Varghese,
Lekshmi J. Das,
Priya Prabhakaran,
Ashwini M. Ananda,
Reeta S. Mani,
Easwaran Sreekumar

Published: April 10, 2025
https://doi.org/10.1371/journal.pntd.0013010

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