Optimization of TaqMan-based quantitative PCR diagnosis for Entamoeba histolytica using droplet digital PCR

• Akira Kawashima,

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Validation, Visualization, Writing – original draft

  Affiliations AIDS Clinical Center, National Center for Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan, The Joint Research Center for Human Retrovirus Infection Kumamoto University Campus, Kumamoto, Japan, Department of Parasitology, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan

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• Yasuaki Yanagawa,

  Roles Data curation, Project administration, Writing – review & editing

  Affiliations AIDS Clinical Center, National Center for Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, United States of America

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• Takayuki Chikata,

  Roles Validation, Writing – review & editing

  Affiliation The Joint Research Center for Human Retrovirus Infection Kumamoto University Campus, Kumamoto, Japan

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• Rieko Shimogawara,

  Roles Formal analysis, Investigation, Writing – review & editing

  Affiliation Department of Parasitology, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan

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• Daisuke Mizushima,

  Roles Supervision, Writing – review & editing

  Affiliations AIDS Clinical Center, National Center for Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan, The Joint Research Center for Human Retrovirus Infection Kumamoto University Campus, Kumamoto, Japan

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• Kiyoto Tsuchiya,

  Roles Supervision, Writing – review & editing

  Affiliation AIDS Clinical Center, National Center for Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan

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• Kenji Yagita,

  Roles Formal analysis, Investigation, Writing – review & editing

  Affiliation Department of Parasitology, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan

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• Hiroyuki Gatanaga,

  Roles Supervision, Writing – review & editing

  Affiliations AIDS Clinical Center, National Center for Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan, The Joint Research Center for Human Retrovirus Infection Kumamoto University Campus, Kumamoto, Japan

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• Koji Watanabe

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: kwatanab@tokai.ac.jp

  Affiliations The Joint Research Center for Human Retrovirus Infection Kumamoto University Campus, Kumamoto, Japan, Department of Parasitology, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan, Division of Host Defense Mechanism, Tokai University School of Medicine, Kanagawa, Japan

  https://orcid.org/0000-0002-3190-8439

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