Establishment of an RPA-CRISPR/Cas12a combined diagnostic system for Pneumocystis jirovecii pneumonia

Yun Wu; Yuhan Shao; Wei Li; Ying Yu; Xia Rao; Jingyi Li; Nicholas R. Waterfield; Guowei Yang

doi:10.1371/journal.pntd.0012922

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Open Access

Peer-reviewed

Research Article

Establishment of an RPA-CRISPR/Cas12a combined diagnostic system for Pneumocystis jirovecii pneumonia

Yun Wu,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Software, Visualization, Writing – original draft, Writing – review & editing

Affiliation Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China
⨯
Yuhan Shao,

Roles Data curation, Investigation, Methodology, Resources

Affiliation Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China
⨯
Wei Li,

Roles Data curation, Investigation, Methodology, Resources

Affiliation Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China
⨯
Ying Yu,

Roles Data curation, Investigation, Methodology, Resources

Affiliation Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China
⨯
Xia Rao,

Roles Investigation, Resources, Validation

Affiliation Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China
⨯
Jingyi Li,

Roles Investigation, Resources, Validation

Affiliation Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China
⨯
Nicholas R. Waterfield,

Roles Formal analysis, Writing – review & editing

Affiliation Warwick Medical School, Warwick University, Coventry, United Kingdom
⨯
Guowei Yang

Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Supervision, Writing – review & editing

* E-mail: yangguowei@hotmail.com

Affiliation Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China

https://orcid.org/0000-0002-3090-6677

⨯

Establishment of an RPA-CRISPR/Cas12a combined diagnostic system for Pneumocystis jirovecii pneumonia

Yun Wu,
Yuhan Shao,
Wei Li,
Ying Yu,
Xia Rao,
Jingyi Li,
Nicholas R. Waterfield,
Guowei Yang

Published: March 18, 2025
https://doi.org/10.1371/journal.pntd.0012922

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