Development and validation of a high-throughput qPCR platform for the detection of soil-transmitted helminth infections

• Nils Pilotte ,

  Contributed equally to this work with: Nils Pilotte, Victor Omballa

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: nils.pilotte@quinnipiac.edu

  Affiliation Department of Biological Sciences, Quinnipiac University, Hamden, Connecticut, United States of America

  https://orcid.org/0000-0002-8447-7425

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• Victor Omballa ,

  Contributed equally to this work with: Nils Pilotte, Victor Omballa

  Roles Conceptualization, Methodology, Writing – original draft, Writing – review & editing

  Affiliations Department of Global Health, University of Washington, Seattle, Washington, United States of America, DeWorm3 Project, Seattle, Washington, United States of America, Quantigen, Fishers, Indiana, United States of America

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• Monica Voss,

  Roles Formal analysis, Investigation, Methodology, Project administration, Validation, Writing – review & editing

  Affiliation Quantigen, Fishers, Indiana, United States of America

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• Leah Padgett,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing – review & editing

  Affiliation Quantigen, Fishers, Indiana, United States of America

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• Malathi Manuel,

  Roles Investigation, Project administration, Validation, Writing – review & editing

  Affiliation The Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College, Vellore, India

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• Jeanne L. Goodman,

  Roles Project administration, Resources, Writing – review & editing

  Affiliations Department of Global Health, University of Washington, Seattle, Washington, United States of America, DeWorm3 Project, Seattle, Washington, United States of America

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• Tim Littlewood,

  Roles Conceptualization, Funding acquisition, Writing – review & editing

  Affiliation Department of Life Sciences, Natural History Museum, London, United Kingdom

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• Zayina Zondervenni Manoharan,

  Roles Investigation, Writing – review & editing

  Affiliation The Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College, Vellore, India

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• Lisette van Lieshout,

  Roles Data curation, Formal analysis, Methodology, Software, Writing – review & editing

  Affiliation Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands

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• Jaco J. Verweij,

  Roles Conceptualization, Methodology, Writing – review & editing

  Affiliation Microvida, Laboratory for Medical Microbiology and Immunology, St. Elisabeth Ziekenhuis, Tilburg, The Netherlands

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• Manigandan Sekar,

  Roles Investigation

  Affiliation The Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College, Vellore, India

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• Ajith Kumar Muthukumar,

  Roles Investigation

  Affiliation The Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College, Vellore, India

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• Gretchen Walch,

  Roles Investigation, Methodology, Validation, Writing – review & editing

  Affiliation Quantigen, Fishers, Indiana, United States of America

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• Abriana Warren,

  Roles Investigation, Methodology, Validation, Writing – review & editing

  Affiliation Quantigen, Fishers, Indiana, United States of America

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• Mariyam Sheikh,

  Roles Investigation, Visualization

  Affiliation Department of Global Health, University of Washington, Seattle, Washington, United States of America

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• Andrew Gonzalez,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Department of Biological Sciences and Biochemistry, Smith College, Northampton, Massachusetts, United States of America

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• Sean R. Galagan,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing

  Affiliations Department of Global Health, University of Washington, Seattle, Washington, United States of America, DeWorm3 Project, Seattle, Washington, United States of America

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• Sitara Swarna Rao Ajjampur,

  Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliation The Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College, Vellore, India

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• Moudachirou Ibikounlé,

  Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliations Centre de Recherche pour la lutte contre les Maladies Infectieuses Tropicales (CReMIT/TIDRC), Université d’Abomey-Calavi, Abomey-Calavi, Bénin, Institut de Recherche Clinique du Bénin, Abomey-Calavi, Bénin

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• Steven A. Williams,

  Roles Conceptualization, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliation Department of Biological Sciences and Biochemistry, Smith College, Northampton, Massachusetts, United States of America

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• Doug Rains,

  Roles Formal analysis, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

  Affiliation Quantigen, Fishers, Indiana, United States of America

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• Ushashi Dadwal,

  Roles Formal analysis, Investigation, Methodology, Validation, Writing – review & editing

  Affiliation Quantigen, Fishers, Indiana, United States of America

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•  [ ... ],
• Judd L. Walson

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliations DeWorm3 Project, Seattle, Washington, United States of America, Departments of Global Health, Medicine (Infectious Disease), Pediatrics and Epidemiology, University of Washington, Seattle, Washington, United States of America, Department of International Health, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America

  https://orcid.org/0000-0003-4836-720X

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