Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker

Saskia du Pré; Mickey Konings; Dorenda J. A. Schoorl; Ahmed H. Fahal; Mark Arentshorst; Arthur F. J. Ram; Wendy W. J. van de Sande

doi:10.1371/journal.pntd.0012092

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Open Access

Peer-reviewed

Research Article

Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker

Saskia du Pré,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Visualization, Writing – original draft, Writing – review & editing

Affiliation Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
⨯
Mickey Konings,

Roles Formal analysis, Investigation, Methodology, Visualization

Affiliation Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands

https://orcid.org/0000-0003-3983-4304

⨯
Dorenda J. A. Schoorl,

Roles Formal analysis, Investigation, Methodology, Visualization

Affiliation Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands

https://orcid.org/0009-0008-2445-2972

⨯
Ahmed H. Fahal,

Roles Resources

Affiliation Mycetoma Research Centre, Khartoum, Sudan

https://orcid.org/0000-0003-4922-7321

⨯
Mark Arentshorst,

Roles Formal analysis, Methodology, Resources, Supervision, Writing – review & editing

Affiliation Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands

https://orcid.org/0000-0001-7693-9370

⨯
Arthur F. J. Ram,

Roles Conceptualization, Methodology, Resources, Supervision, Writing – review & editing

Affiliation Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands

https://orcid.org/0000-0002-2487-8016

⨯
Wendy W. J. van de Sande

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: w.vandesande@erasmusmc.nl

Affiliation Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands

https://orcid.org/0000-0002-8652-8783

⨯

Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker

Saskia du Pré,
Mickey Konings,
Dorenda J. A. Schoorl,
Ahmed H. Fahal,
Mark Arentshorst,
Arthur F. J. Ram,
Wendy W. J. van de Sande

Published: April 5, 2024
https://doi.org/10.1371/journal.pntd.0012092

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