Validation of the performance of a point of care molecular test for leprosy: From a simplified DNA extraction protocol to a portable qPCR

• Amanda Bertão-Santos,

  Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft

  Affiliation Laboratório de Ciências e Tecnologias Aplicadas à Saúde (LaCTAS), Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Curitiba, Brazil

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• Larisse da Silva Dias,

  Roles Investigation, Methodology

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil

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• Marcelo Ribeiro-Alves,

  Roles Formal analysis, Writing – review & editing

  Affiliation Laboratório de Pesquisa Clínica em DST e AIDS, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil

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• Roberta Olmo Pinheiro,

  Roles Funding acquisition, Project administration, Resources, Writing – review & editing

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil

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• Milton Ozório Moraes †,

  † Deceased.

  Roles Conceptualization, Funding acquisition, Project administration, Resources

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil

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• Fernanda Saloum de Neves Manta ,

  Contributed equally to this work with: Fernanda Saloum de Neves Manta, Alexandre Dias Tavares Costa

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: alexandre.costa@fiocruz.br, adtcosta@gmail.com; femanta@yahoo.com.br

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil

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• Alexandre Dias Tavares Costa

  Contributed equally to this work with: Fernanda Saloum de Neves Manta, Alexandre Dias Tavares Costa

  Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Writing – review & editing

  * E-mail: alexandre.costa@fiocruz.br, adtcosta@gmail.com; femanta@yahoo.com.br

  Affiliation Laboratório de Ciências e Tecnologias Aplicadas à Saúde (LaCTAS), Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Curitiba, Brazil

  https://orcid.org/0000-0001-8434-2152

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