Optimized strategy for real-time qPCR detection of Onchocerca volvulus DNA in pooled Simulium sp. blackfly vectors

• Mary Doherty ,

  Roles Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: mdoherty82@smith.edu

  Affiliation Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America

  https://orcid.org/0009-0003-3689-9628

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• Jessica R. Grant,

  Roles Formal analysis, Investigation, Methodology, Visualization, Writing – original draft

  Affiliation Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America

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• Nils Pilotte,

  Roles Conceptualization, Formal analysis, Methodology, Writing – original draft, Writing – review & editing

  Affiliation Department of Biological Sciences, Quinnipiac University, Hamden, Connecticut, United States of America

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• Sasisekhar Bennuru,

  Roles Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

  Affiliation Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, United States of America

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• Kerstin Fischer,

  Roles Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

  Affiliation Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America

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• Peter U. Fischer,

  Roles Conceptualization, Formal analysis, Resources, Writing – original draft, Writing – review & editing

  Affiliation Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America

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• Sara Lustigman,

  Roles Conceptualization, Investigation, Methodology, Resources, Writing – original draft, Writing – review & editing

  Affiliation Laboratory of Molecular Parasitology, Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, United States of America

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• Thomas B. Nutman,

  Roles Conceptualization, Resources, Writing – original draft, Writing – review & editing

  Affiliation Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, United States of America

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• Kenneth Pfarr,

  Roles Formal analysis, Investigation, Methodology, Resources, Writing – original draft, Writing – review & editing

  Affiliations Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany, German Center for Infection Research (DZIF), Partner-Site Bonn-Cologne, Bonn, Germany

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• Achim Hoerauf,

  Roles Resources, Writing – original draft, Writing – review & editing

  Affiliations Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany, German Center for Infection Research (DZIF), Partner-Site Bonn-Cologne, Bonn, Germany

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• Thomas R. Unnasch,

  Roles Conceptualization, Resources, Writing – original draft, Writing – review & editing

  Affiliation Center for Global Health Infectious Disease Research, University of South Florida, Tampa, Florida, United States of America

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• Hassan K. Hassan,

  Roles Investigation, Methodology, Resources, Writing – original draft, Writing – review & editing

  Affiliation Center for Global Health Infectious Disease Research, University of South Florida, Tampa, Florida, United States of America

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• Samuel Wanji,

  Roles Resources, Writing – original draft, Writing – review & editing

  Affiliations Parasite and Vectors Research Unit, Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon, Research Foundation in Tropical Diseases and the Environment, Buea, Cameroon

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• Patrick J. Lammie,

  Roles Conceptualization, Funding acquisition, Writing – original draft, Writing – review & editing

  Affiliation NTD-SC, Task Force for Global Health, Atlanta, Georgia, United States of America

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• Eric Ottesen,

  Roles Conceptualization, Funding acquisition, Writing – original draft, Writing – review & editing

  Affiliation NTD-SC, Task Force for Global Health, Atlanta, Georgia, United States of America

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• Charles Mackenzie,

  Roles Conceptualization, Funding acquisition, Writing – original draft, Writing – review & editing

  Affiliations NTD-SC, Task Force for Global Health, Atlanta, Georgia, United States of America, RLMF, The END Fund, New York, New York, United States of America

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•  [ ... ],
• Steven A. Williams

  Roles Conceptualization, Formal analysis, Funding acquisition, Resources, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America, Molecular and Cellular Biology Program, University of Massachusetts, Amherst, Massachusetts, United States of America

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