Simplified molecular diagnosis of visceral leishmaniasis: Laboratory evaluation of miniature direct-on-blood PCR nucleic acid lateral flow immunoassay

• Norbert J. van Dijk ,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Writing – original draft

  * E-mail: n.j.vandijk@amsterdamumc.nl

  Affiliations Amsterdam University Medical Centre, Department of Medical Microbiology and Infection Prevention, Experimental Parasitology, Amsterdam, the Netherlands, Amsterdam Institute for Infection and Immunity, Infectious Diseases Programme, Amsterdam, the Netherlands

  https://orcid.org/0000-0003-4747-090X

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• Dawit Gebreegziabiher Hagos,

  Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft

  Affiliations Amsterdam University Medical Centre, Department of Medical Microbiology and Infection Prevention, Experimental Parasitology, Amsterdam, the Netherlands, Amsterdam Institute for Infection and Immunity, Infectious Diseases Programme, Amsterdam, the Netherlands, College of Health Sciences, School of Medicine, Department of Medical Microbiology and Immunology, Mekelle University, Mekelle, Ethiopia

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• Daniela M. Huggins,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology

  Affiliation Amsterdam University Medical Centre, Department of Medical Microbiology and Infection Prevention, Experimental Parasitology, Amsterdam, the Netherlands

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• Eugenia Carrillo,

  Roles Conceptualization, Resources, Supervision, Writing – review & editing

  Affiliations WHO Collaborating Centre for Leishmaniasis, National Center for Microbiology, Instituto de Salud Carlos III, Majadahonda (Madrid), Spain, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Madrid, Spain

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• Sophia Ajala,

  Roles Investigation

  Affiliation Amsterdam University Medical Centre, Department of Medical Microbiology and Infection Prevention, Experimental Parasitology, Amsterdam, the Netherlands

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• Carmen Chicharro,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliations WHO Collaborating Centre for Leishmaniasis, National Center for Microbiology, Instituto de Salud Carlos III, Majadahonda (Madrid), Spain, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Madrid, Spain

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• David Kiptanui,

  Roles Investigation

  Affiliation Kacheliba Sub-County Hospital, Kacheliba, West Pokot County, Kenya

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• Jose Carlos Solana,

  Roles Formal analysis, Methodology

  Affiliations WHO Collaborating Centre for Leishmaniasis, National Center for Microbiology, Instituto de Salud Carlos III, Majadahonda (Madrid), Spain, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Madrid, Spain

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• Edwin Abner,

  Roles Investigation

  Affiliation Kacheliba Sub-County Hospital, Kacheliba, West Pokot County, Kenya

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• Dawit Wolday,

  Roles Funding acquisition, Resources, Supervision

  Affiliation Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada

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• Henk D. F. H. Schallig

  Roles Conceptualization, Resources, Supervision, Writing – review & editing

  Affiliations Amsterdam University Medical Centre, Department of Medical Microbiology and Infection Prevention, Experimental Parasitology, Amsterdam, the Netherlands, Amsterdam Institute for Infection and Immunity, Infectious Diseases Programme, Amsterdam, the Netherlands

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