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Open Access

Peer-reviewed

Research Article

Delving in folate metabolism in the parasite Leishmania major through a chemogenomic screen and methotrexate selection

Sophia Bigot,

Roles Data curation, Formal analysis, Methodology, Writing – original draft

Affiliation Centre de Recherche en Infectiologie du Centre de Recherche du CHU de Québec and Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Quebec City, Québec, Canada
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Philippe Leprohon,

Roles Conceptualization, Data curation, Software, Supervision, Validation, Writing – review & editing

Affiliation Centre de Recherche en Infectiologie du Centre de Recherche du CHU de Québec and Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Quebec City, Québec, Canada
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Marc Ouellette

Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

* E-mail: Marc.Ouellette@crchudequebec.ulaval.ca

Affiliation Centre de Recherche en Infectiologie du Centre de Recherche du CHU de Québec and Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Quebec City, Québec, Canada

https://orcid.org/0000-0002-6743-9646

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Delving in folate metabolism in the parasite Leishmania major through a chemogenomic screen and methotrexate selection

Sophia Bigot,
Philippe Leprohon,
Marc Ouellette

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Published: June 29, 2023
https://doi.org/10.1371/journal.pntd.0011458

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Point mutation
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Mutation
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Leishmania
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Transfection
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Genomics
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Substitution mutation
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Dideoxy DNA sequencing
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Hyperexpression techniques
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