A whole blood intracellular cytokine assay optimised for field site studies demonstrates polyfunctionality of CD4+ T cells in acute scrub typhus

• Manutsanun Inthawong,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Software, Validation, Visualization, Writing – original draft

  Affiliations Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Department of Veterinary Medicine, United States Army Medical Directorate, Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS), Bangkok, Thailand

  https://orcid.org/0000-0002-8956-4253

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• Nattapon Pinthong,

  Roles Methodology, Resources

  Affiliation Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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• Areerat Thaiprakhong,

  Roles Resources

  Affiliation Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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• Tri Wangrangsimakul,

  Roles Data curation, Resources

  Affiliations Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, NDM Center for Global Health Research, Nuffield Dept. of Clinical Medicine, University of Oxford, Oxford, United Kingdom

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• Piyanate Sunyakumthorn,

  Roles Resources, Supervision

  Affiliation Department of Veterinary Medicine, United States Army Medical Directorate, Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS), Bangkok, Thailand

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• Jennifer Hill,

  Roles Visualization, Writing – review & editing

  Affiliation NDM Center for Global Health Research, Nuffield Dept. of Clinical Medicine, University of Oxford, Oxford, United Kingdom

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• Piengchan Sonthayanon,

  Roles Supervision, Writing – review & editing

  Affiliations Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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• Daniel H. Paris,

  Roles Conceptualization, Funding acquisition, Writing – review & editing

  Affiliations Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Department of Medicine, Swiss Tropical and Public Health Institute, Allschwil, Switzerland, Department of Clinical Research, University of Basel, Basel, Switzerland

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• Susanna J. Dunachie,

  Roles Conceptualization, Funding acquisition, Investigation, Methodology, Validation, Writing – review & editing

  Affiliations Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, NDM Center for Global Health Research, Nuffield Dept. of Clinical Medicine, University of Oxford, Oxford, United Kingdom

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• Barbara Kronsteiner

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

  * E-mail: barbara.kronsteiner-dobramysl@ndm.ox.ac.uk

  Affiliations Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, NDM Center for Global Health Research, Nuffield Dept. of Clinical Medicine, University of Oxford, Oxford, United Kingdom

  https://orcid.org/0000-0003-0867-2867

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