Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus

• Meijun Guo ,

  Contributed equally to this work with: Meijun Guo, Shanshan Du, Lijin Lai

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft

  Affiliations School of Public Health, the key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, China, NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Shanshan Du ,

  Contributed equally to this work with: Meijun Guo, Shanshan Du, Lijin Lai

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Lijin Lai ,

  Contributed equally to this work with: Meijun Guo, Shanshan Du, Lijin Lai

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft

  Affiliations NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China, Shenzhen Hospital of the University of Chinese Academy of Sciences (Guangming), Shenzhen, China

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• Wei Wu,

  Roles Conceptualization

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Xiaoxia Huang,

  Roles Conceptualization

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Aqian Li,

  Roles Conceptualization

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Hao Li,

  Roles Conceptualization, Investigation

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Chuan Li,

  Roles Conceptualization

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Qin Wang,

  Roles Conceptualization

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Lina Sun,

  Roles Conceptualization

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Tiezhu Liu,

  Roles Investigation

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Tingting Tian,

  Roles Investigation

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Shiwen Wang,

  Roles Conceptualization, Resources

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Mifang Liang,

  Roles Conceptualization, Resources

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Dexin Li,

  Roles Conceptualization, Resources

  Affiliation NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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• Chun Xie ,

  Roles Conceptualization, Supervision, Validation

  * E-mail: 1009207189@qq.com (CX); lijd@ivdc.chinacdc.cn (JL)

  Affiliation School of Public Health, the key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, China

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•  [ ... ],
• Jiandong Li

  Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – review & editing

  * E-mail: 1009207189@qq.com (CX); lijd@ivdc.chinacdc.cn (JL)

  Affiliations School of Public Health, the key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, China, NHC Key Laboratory of Biosafety, China CDC, Beijing, China; NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

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