Smartphone multiplex microcapillary diagnostics using Cygnus: Development and evaluation of rapid serotype-specific NS1 detection with dengue patient samples

• Sarah Helen Needs ,

  Contributed equally to this work with: Sarah Helen Needs, Sirintra Sirivisoot

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Reading School of Pharmacy, University of Reading, Whiteknights, Reading, United Kingdom

  https://orcid.org/0000-0003-3407-9637

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• Sirintra Sirivisoot ,

  Contributed equally to this work with: Sarah Helen Needs, Sirintra Sirivisoot

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Current address: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand

  Affiliation Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

  https://orcid.org/0000-0002-5809-6936

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• Sophie Jegouic,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Reading School of Pharmacy, University of Reading, Whiteknights, Reading, United Kingdom

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• Tanapan Prommool,

  Roles Methodology, Resources

  Affiliation Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand

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• Prasit Luangaram,

  Roles Methodology, Resources

  Affiliation Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand

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• Chatchawan Srisawat,

  Roles Methodology, Resources

  Affiliation Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

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• Kanokwan Sriraksa,

  Roles Methodology, Resources

  Affiliation Pediatric Department, Khon Kaen Hospital, Ministry of Health, Khon Kaen, Thailand

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• Wannee Limpitikul,

  Roles Methodology, Resources

  Affiliation Pediatric Department, Songkhla Hospital, Ministry of Health, Songkhla, Thailand

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• Dumrong Mairiang,

  Roles Formal analysis, Writing – review & editing

  Affiliations Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

  https://orcid.org/0000-0002-2743-0145

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• Prida Malasit,

  Roles Methodology, Resources

  Affiliations Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

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• Panisadee Avirutnan ,

  Roles Conceptualization, Funding acquisition, Methodology, Resources, Supervision, Visualization, Writing – review & editing

  * E-mail: panisadee.avi@mahidol.edu (PA); chunyapk@biotec.or.th (CH); a.d.edwards@reading.ac.uk (ADE)

  Affiliations Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

  https://orcid.org/0000-0003-0053-3045

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• Chunya Puttikhunt ,

  Roles Conceptualization, Funding acquisition, Methodology, Resources, Supervision, Visualization, Writing – review & editing

  * E-mail: panisadee.avi@mahidol.edu (PA); chunyapk@biotec.or.th (CH); a.d.edwards@reading.ac.uk (ADE)

  Affiliations Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

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• Alexander Daniel Edwards

  Roles Conceptualization, Funding acquisition, Methodology, Resources, Supervision, Visualization, Writing – review & editing

  * E-mail: panisadee.avi@mahidol.edu (PA); chunyapk@biotec.or.th (CH); a.d.edwards@reading.ac.uk (ADE)

  Affiliations Reading School of Pharmacy, University of Reading, Whiteknights, Reading, United Kingdom, Capillary Film Technology Ltd, Billingshurst, West Sussex, United Kingdom

  https://orcid.org/0000-0003-2369-989X

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